Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence. This 4-day course gives you a step by step approach into flow cytometry technique, taking the time to integrate and apply the most important principles of the technology. It is particularly designed for beginners or self-trained users. The program contains lectures, exercises, hands-on sessions, and data analysis. The course will include tutorials on the theory and practice of all the techniques and methods covered. Laboratory sessions will address sample preparation, instrument construction and operation, data collection and analysis, 

For further information you can study:
http://www.d.umn.edu/~biomed/flowcytometry/introflowcytometry.pdf

Day 1:

Basics  History, importance
 What Is FlowCytometry?
 Instrumentation: Into the Black Box
 Flow Cytometric Measurements
 Data Acquisition
 Data Storage
 Data Analysis
 FACS
 Information Recourses
Day 2: DNA assays Cell Cycle, Apoptosis, Live-Dead
Day 3: protein analyses Cell surface protein quantification
Day 4: Efflux assays Drug resistance evaluation